0.2ml isiri-skirted pcl 96-zvakanaka mahwendefa

0.2ml isiri-skirted pcr 96 zvakanaka mahwendefa

PCR 96 Plates zvakakosha zvishandiso zvinoshandiswa mumanyorero ebiology kune dzakasiyana-siyana zvikumbiro, kunyanya mukuwedzera kweDNA kuburikidza nePolymerase Chain (PCR). Heano mafaro makuru:

1. Dna Amplization:
Zvikuru zvinoshandiswa kukwikwidza Sampuli Sampuli mune yakakwira-incurity inonyorera, kubvumira akawanda samples kuti igadziriswe panguva imwe chete.

2. Quantitative PCR (QPCR):
Yakanakira chaiyo-nguva kuwanda PCR, inogonesa iyo kuiswa kweDNA kana rna mune sampula uchishandisa fluorescent dhayi kana probes.

3. Genotyping:
Akashanda muGenotyping zvidzidzo kuti aongorore genetic akasiyana mumigwagwa yakawanda.

4. Clone kuongorora:
Inoshanda kune kuongorora mawindo mumanyorero ekuedza, achibvumira vaongorori kuti vasimbise kuvapo kwemhepo.

5. Mutagensis fundo:
Inoiswa muzvidzidzo zvinosanganisira saiti-inotungamirwa Mutagenesis kuongorora zvinokonzerwa nekuchinja chaiko paGene Basa.

6. Yakakwira-yekuburitsa kuongorora:
Inofambisa yakakwirira-yekuchina assays, ichiita kuti iite yakakodzera kuwanikwa kwezvinodhaka uye zvimwe zvinoshandiswa zvinoda kuongororwa kwemazhinji samples.

7. Sampuli yekuchengetedza:
Inogona kushandiswa pakuchengetedza kweDNA sampuli kana maitiro emabasa ekutevera ongororo.